Recent Posts

The intention of this post is to give a short introduction into the topic of read composition, which is an important feature of NGS sequencing analysis. Quality value – what are they and why do I need them? Reads received from e.g. Illuminas Sequencing Machines do not only contain the corresponding nucleotides, but...

The intention of this post is to help you using our GC-content tool TrueGC which will give you information about the GC-content of the NGS sequences you have. What you need to use TrueGC To use TrueGC, you will need sequences. You can use those sequences which were directly given by the next generation sequencing...

This blog post looks at some of the benefits of UV decontamination as well as its limitations for PCR work. The principal sources of contamination are aerosolized PCR products that settle on surfaces, equipment and consumables which are then used for PCR. Cleaning flat surfaces with strong oxidizers such as NaOCl or...

The intention of this post is to help you using our evenness tool TruePlane which will give you an idea about the evenness of the NGS sequences you have. What you need to use TruePlane To use TruePlane, you will need sequences. You can use those sequences which were directly given by the next generation sequencing...

The intention of this post is to give a short introduction into the topic of evenness of coverage, which is an important feature of a sequence alignment.   Evenness – Why should I check this? In NGS (whole genome sequencing/whole exome sequencing/amplicon sequencing) you normally get reads or sequences from a...